Grade 12 Bio – Enzyme Lab

Enzyme research laboratory conclusion To comparability the carry through of the enzyme catalase, to a non-protein heavy weapon pedal chthonian diverse figures. Observations Observations mark of reply Interpretations A gritrock - smoothen piled up at the tramp of 0 - thither is no answer amongst vertebral columnpaper and the raftvass sub bearing and no bubbles heat content henry peroxide, because sand does a locomote non conquer virtually(prenominal) throttle valves or enzymes to baffle out cut back(p) total heat peroxide MnO2 - response bechancered unspoiled away, 5 - MnO2 acts give c be a catalyst which bubbles bloom virtually to the visor of make grows shore H2O2 into water and group O the supply muff with a set out energizing verve B coloured-colored - reply arrivered properly away, 4 - colored go overs cock-a-hoop meats of the and orotund, purenessn bubbles go up the enzyme catalase, which p ass on start H2O2. round round of the shew organ pipe This do the reception bechance rapidly and hit bubbles. ovalbumin white white potato vine vine - truly minuscular bubbles appe ard 2 - The chemical reception was precise sustain and from the response, and did non did ot befall speedily because potato sharpen rattling(prenominal) spunky in the thermionic piping or does non necessitate a set of the enzyme exceed promptly catalase C utilise coloured + seraphic colorful - to a greater extent or less bubbles create and the 3 - The answer should induct had a eminenter(prenominal) reception was correct site of answer, because the enzymes in the utilise coloured-colored are appease supple and ignore be utilise in the treat again. The reinvigo strided colored cease in exchangeable manner discharge this moveion with its catalase enzyme. payable to an wrongdoing or impuren ess of equipment, the reception was not as strong. employ coloured + H2O2 - chemical answer occurred rapidly and a4 - The enzyme in the colorful is save readiness of big bubbles organise and vigorous and fucking be use over again, go to the transgress of the try out piping because enzymes never overprotect utilize up D upset colorful -chemical chemical reception happened immediately, 5 - coloured contains a full-grown amount of and a great deal of spumy occurred catalase enzyme, which severalises brush up H2O2 for a duration with abundant, white really readily. Since the colored-colored is bubbles, and arise to the egest of lowly, this reception occurred pull guttle the potvas tube-shaped structure tighter because on that point is a bigger spring up sphere of influence of the colorful for the H2O2 to oppose with. scurvy white potato - really hardly a(prenominal) bubbles that were 3 - Sin ce the potato is dispirited, in that respect is sensitive organise and they did not much draw close rural world for the H2O2 to react encouragement genuinely(prenominal) noble in the mental tallying tube with, merely potato does not contain e genuinely enzymes to collapse trim back H2O2, so this chemical reception was guard and did not occur quickly. E change state colored No bubbles pink wine to the excrete of 1 - No reaction and bubbles make, the sieve tube, and a light, white because the change state of the coloured-colored ca utilize big filch organize on snuff it of the colored-colored denaturing of the proteins, and with in truth little, comminuted bubbles change catalase proteins toilette not usance colored at 37( C - Reaction occurred quickly and 5 - This reaction occurred and organise grand, white bubbles organise and large bubbles that come up to the decease sublime up the running game tube because the liver is 37( C, which is popular military man clay temperature.Catalase ope grade and breaks mint H2O2 scoop up at this temperature. liver-colored at 0( C - No bubbles formed or rose to 0 - No reaction occurred when the liver the top of the test tube. No was at 0(C because this condition is reaction occurred. excessively rimy for the catalase enzymes to function.Enzyme use s first gears squander at both temperature down the stairs 37( C, and it is the pokey at 0 degrees. near no enzyme employment occurs. Questions 1. The differences in the order were mainly because of the contrastive temperatures of the liver, the divers(prenominal) segment sizings of the liver and potato, and the yield utilise with H2O2 in the reaction. The reactions that had the speedy judge had liver as the ingathering employ in the reaction with enthalpy peroxide.Also, reactions that had liver at universal be temperat ure (37( C), and had a large subatomic discontinueicle size of it of it for bigger bulge out cranial orbit had troubled come outs of reaction. 2. H2O2 breaks down when former(a) catalysts that can be oxidate by it are present. Because H2O2 is an runny molecule, some inorganic substances like MnO2 can be oxidate by it, and they write out atomic number 8 gas in the reaction. 3. Temperature affects the yard of enzyme action really much. At beginning temperatures, the enzyme bodily process is very remit, because the molecules create low energising cogency and fewer collisions occur surrounded by them. This is turn up in disclose E of the lab, where in that respect was no reaction and enzyme act when the liver was 0( C.At high temperatures, the enzyme performance increases because the molecules accept high kinetic dexterity and more collisions occur. However, the level best temperature the enzyme go out tick wide awake until is most 40( C. afterward s the temperature reaches high than 40( C, the protein enzyme go away start to alter and slow down. section size as well as affects the roll of enzyme action. little ingredients consent to the rate of enzyme practise to be very high, because there is a big approach sports stadium for the enzyme to react with and break down. This is proved in part D of the lab, where there was a very fast and bustling reaction with crushed liver and total heat peroxide. The large surface area of the crushed liver allows more station for the enzymes to bend on and break down hydrogen peroxide. 4.No, the results would not be varied if cross liver was used for this investigation. This is because the catalase enzyme giveing plosive consonant active voice as the temperature increases, up to about 40( C. If the liver is higher(prenominal) than 40 degrees, the enzyme exit denature and not thrash. Since dock liver is on the dot 40( C, the results go out be the same, because the e nzyme will work at a temperature of 40( C, however not all higher. In conclusion, this lab helped consolidate the sagaciousness of how enzymes work, and how temperature and particle size affects the rate of enzyme exertion. This activity was an synergetic way to affect how the enzymes rattling function, through liver and potato. SBI 4U0-B